Thank you for using Adaptive’s immunoSEQ Assay in your research. When preparing a manuscript, we strongly suggest referencing the description of our methods and publications as listed below. If you have additional questions regarding how your samples were processed or if you would like assistance with a manuscript, please contact us at email@example.com, and one of our research scientists will be happy to assist you.
Description of Methods and Publications
Amplification and sequencing of [TCRB/IGH/IGKL/TCRAD/TCRG] CDR3 was performed using the immunoSEQ Platform (Adaptive Biotechnologies®, Seattle, WA). The immunoSEQ Platform combines multiplex PCR with high throughput sequencing and a sophisticated bioinformatics pipeline for [TCRB/IGH/IGKL/TCRAD/TCRG] CDR3 analysis.1,2
1. Comprehensive assessment of T-cell receptor β-chain diversity in αβ T cells. Blood, Nov 2009. Robins HS, Campregher PV, Srivastava SK, Wacher A, Turtle CJ, Kahsai O, Riddell SR, Warren EH, Carlson CS.
2. Using synthetic templates to design an unbiased multiplex PCR assay. Nature Communications, October 2013. Christopher S Carlson, Ryan O Emerson, Anna M Sherwood, Cindy Desmarais, Moon-Wook Chung, Joseph M Parsons, Michelle S Steen, Marissa A LaMadrid-Herrmannsfeldt, David W Williamson, Robert J Livingston, David Wu, Brent L Wood, Mark J Rieder & Harlan Robins.