Accuracy and quantification by design
immunoSEQ Assays utilize a multiplex PCR-based assay enabling us to sequence directly from genomic DNA. We provide precise, quantitative abundance data on your cell populations, unlike the relative abundance or variable expression data of RNA-based assays. Each immunoSEQ assay contains synthetic immune templates as in-line controls that ensure accurate, quantitative, and unbiased results with batch-to-batch consistency.
First, a synthetic immune repertoire was designed to represent all VJ-gene combinations.
Then, measuring against our synthetic repertoire, we were able to adjust our primer concentrations to significantly reduce amplification bias.
In-line controls for every sample, enable us to measure amplification, correct residual bias & provide absolute counts of the immune cells in your sample.
Sophisticated bioinformatics correct errors & collapse sequences to deliver highly accurate, high-volume, standardized data sets.
Read the full story in Nature Communications:
“Using synthetic templates to design an unbiased multiplex PCR assay”