TECHNICAL SUPPORT

immunoSEQ resources & support

Manuals and Guidelines

Target Starting Quantities for Extracting DNA
Listing of sample types and target starting quantities for extracting DNA to reach the sample amounts outlined in the Service Sample guidelines.

Sample Packaging & Shipping Instructions

Sample Guidelines – Human T Cell

Sample Guidelines – Human B Cell

Sample Guidelines – Mouse T and B Cell

Understanding the immunoSEQ Assay
An introduction to sequencing the immune repertoire and an overview of the types of results generated by the immunoSEQ Assay.

Grant Writing Support Materials
Download ready-to-go assay-specific descriptions of immunoSEQ methods and publication references, and general product descriptions to use when completing funding proposals.

>T-cell Receptor Methods: Human and Mouse assays

>B-cell Receptor Methods: Human and Mouse assays

>V(D)J Recombination Chart Image

FAQS

Yes, access to the Analyzer’s full functionality is available to Service and Kit customers as well as anyone interested in analyzing the open-access immunoSEQ Data available in immuneACCESS™.

There are many factors to take into consideration when deciding between our immunoSEQ RUO Kit and Service products. With our immunoSEQ Service, we send you a sample box to submit your samples, and our expert technicians will sequence your samples in our state-of-the-art lab. Our RUO Kit offers you the same quantitative bias-controlled multiplex PCR assay for the human TCRB loci, but you prepare the libraries and do the sequencing yourself, giving you more flexibility and control over your project.

  • Which loci are you interested in?
    • Service: Human TCRB, TCRG, IGH and Mouse TCRB
    • Kit: Human TCRB
  • What sample types are you working with?
    • Our Service lab can process gDNA, cDNA, or FFPE samples.
    • Our Kit supports gDNA samples.
  • Experimental design:
    • When choosing between Adaptive’s RUO hsTCRB immunoSEQ Kit and the hsTCRB Service, consider the full scope of the project. The Kit can process up to 47 tissue or clonal T-cell samples (Surveys), or up to 15 PBMC or peripheral immune repertoire samples (Deeps).
  • Time lines: Our Kit affords more turn-around time flexibility, since you control the timing of the processing and sequencing.
  • Bioinformatics for data analysis:
    • Data generated from Kit and Service orders are processed in the same bioinformatics pipeline. Full access to the powerful data analysis tool in the immunoSEQ Analyzer are included at no additional charge.
  • Budget:
    • Our Kit is the most economical option and allows you the flexibility to run as many or few samples at a time as you wish. Our Service orders require a minimum of 6 samples for the Survey resolution or 3 samples for the Deep resolution.

The immunoSEQ Assays sequence the TCRB, TCRG, and IGH for human samples and TCRB for mouse samples. The immunoSEQ RUO kit is available for the human TCRB locus.

Both the immunoSEQ Service and immunoSEQ Kit include access to the immunoSEQ Analyzer. The Analyzer is a powerful cloud-based software suite that lets you view, analyze, compare and share your immunosequencing data with just a few simple clicks of a mouse. The Analyzer makes it easy to explore your data, and organize and share projects with individuals, groups or as public data sets.

We have a library of Analyzer video tutorials to get you up to speed quickly on the functionality and features of the Analyzer. You can also see entire immunoSEQ data sets in immuneACCESS, our open-access database.

Using sorted cells as a starting material is a good choice when you’re interested in studying a particularly rare sub-set of cells, such as a T-regulatory population. If you were to sequence PBMCs without sorting, the population of Tregs might be too dilute to be identified in the sample without sorting first. If, however, you are interested in looking at T-cell density in a tumor, it’s important to sequence bulk tumor tissue.

Our Account Executives, Scientific Liaisons, and Technical Support teams are happy to work with you to help design a rigorous experiment. Contact our Technical Support team for more information.

Yes. Samples can be shipped to our lab for processing from international locations. Please contact Customer Care for assistance.

Contact Customer Care and they will put you in touch with your Account Executive.

First, verify that you are using the same email address that you used to set up your account, then try resetting your password. Click on the Forgot Password link at the login prompt. This will re-initiate the validation email for your account.

immunoSEQ Assays are for Research Use Only. Not for use in diagnostic procedures.

STILL HAVE QUESTIONS?

Contact technical support

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