TCR repertoire sequencing identifies synovial Treg cell clonotypes in the bloodstream during active inflammation in human arthritis

Maura Rossetti1,2,3, Roberto Spreafico1,2,4, Alessandro Consolaro5, Jing Yao Leong1,Camillus Chua1, Margherita Massa6, Suzan Saidin1, Silvia Magni-Manzoni7,Thaschawee Arkachaisri8, Carol A Wallace9, Marco Gattorno5, Alberto Martini5,Daniel J Lovell10, Salvatore Albani1 1SingHealth Translational Immunology and Inflammation Centre, SingHealth and Duke-NUS Graduate Medical School, Singapore, Singapore, 2Translational Research Unit, Sanford-Burnham Medical Research Institute, San Diego, California, USA, 3Department of Pathology and Laboratory Medicine, University of California Los Angeles,Los Angeles, California, USA, 4Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA, 5Second Pediatrics Division, University of Genoa and G Gaslini Institute, Genova, Italy, 6Lab Biotecnologie, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy, 7Pediatric Rheumatology Unit, IRCCS Ospedale Pediatrico Bambino Gesù, Rome, Italy, 8Duke-NUS Graduate Medical School and Rheumatology and Immunology Service, KK Women's and Children's Hospital, Singapore, Singapore, 9Seattle Children's Hospital and Research Institute, Seattle, Washington, USA, 10Division of Rheumatology, Cincinnati Children's Hospital Medical Center, and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA