TCR Repertoire Intratumor Heterogeneity in Localized Lung Adenocarcinomas: An Association with Predicted Neoantigen Heterogeneity and Postsurgical Recurrence

Alexandre Reuben1,2, Rachel Gittelman3, Jianjun Gao4, Jiexin Zhang5, Erik C. Yusko3, Chang-Jiun Wu2, Ryan Emerson3, Jianhua Zhang2, Christopher Tipton3, Jun Li2, Kelly Quek2,6, Vancheswaran Gopalakrishnan1, Runzhe Chen2,6, Luis M. Vence7, Tina Cascone6, Marissa Vignali3, Junya Fujimoto8, Jaime Rodriguez-Canales8, Edwin R. Parra8, Latasha D. Little2, Curtis Gumbs2, Marie-Andrée Forget9, Lorenzo Federico9, Cara Haymaker9, Carmen Behrens8, Sharon Benzeno3, Chantale Bernatchez9, Boris Sepesi10, Don L. Gibbons6, Jennifer A. Wargo1,2, William N. William Jr6, Stephen Swisher10, John V. Heymach6, Harlan Robins3,11, J. Jack Lee12, Padmanee Sharma4,7, James P. Allison7, P. Andrew Futreal2, Ignacio I. Wistuba8, and Jianjun Zhang2,6 1Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas. 2Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas. 3Adaptive Biotechnologies, Seattle, Washington. 4Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas. 5Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas. 6Department of Thoracic/Head and Neck Medical Oncology, The Univer- sity of Texas MD Anderson Cancer Center, Houston, Texas. 7Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, Texas. 8Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas. 9Department of Melanoma Medical Oncology, The University of Texas MD Ander- son Cancer Center, Houston, Texas. 10Department of Thoracic Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas. 11Department of Computational Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington. 12Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, Texas.